Inntags: small self-structured epitopes for innocuous protein tagging.

TitleInntags: small self-structured epitopes for innocuous protein tagging.
Publication TypeJournal Article
Year of Publication2015
AuthorsGeorgieva, Maya V., Yahya Galal, Codó Laia, Ortiz Raúl, Teixidó Laura, Claros José, Jara Ricardo, Jara Mònica, Iborra Antoni, Gelpí Josep-Lluis, Gallego Carme, Orozco Modesto, and Aldea Martí
JournalNat Methods
Date Published2015 Oct
KeywordsAnimals, Antibodies, Epitopes, Female, Fluorescent Antibody Technique, Green Fluorescent Proteins, HEK293 Cells, Humans, Immunoprecipitation, Inbred BALB C, Mice, Monoclonal, NIH 3T3 Cells, Protein Conformation, Protein Structure, Proteins, Recombinant Proteins, Tertiary

Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.